monarch spin pcr and dna cleanup kit (New England Biolabs)
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Monarch Spin Pcr And Dna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1452 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+cleanup+kit/Monarch+Spin+PCR+%26+DNA+Cleanup+Kit/pmc13010419-14-0-10
Average 99 stars, based on 1452 article reviews
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Amplification:Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation Article Snippet: Excess primers were removed by treating 50 μl PCR reactions with 3 μl of thermolabile exonuclease 1 (NEB, M0568L) for 4 min at 37°C before heat inactivating for 1 min at 80°C. .. Amplified DNA was then purified using a Monarch Spin PCR and Purification:Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation Article Snippet: Excess primers were removed by treating 50 μl PCR reactions with 3 μl of thermolabile exonuclease 1 (NEB, M0568L) for 4 min at 37°C before heat inactivating for 1 min at 80°C. .. Amplified DNA was then purified using a Monarch Spin PCR and Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs Article Snippet: .. For next generation sequencing (NGS), regions of interest were PCR-amplified and purified using a PCR and Article Title: Molecular insights into the dual activation of PomZ, a ParA/MinD P-loop ATPase, by the two ATPase-activating proteins PomX and PomY. Article Snippet: .. After purification with a Monarch Spin PCR and Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs. Article Snippet: .. For next generation sequencing (NGS), regions of interest were PCRamplified and purified using a PCR and Article Title: Integration of ploidy, Orc1/Cdc6 homolog function, and lysine acetylation with phosphate limitation and UV stress responses of Haloferax volcanii. Article Snippet: .. PCR products of expected size were purified using Monarch PCR and Polymerase Chain Reaction:Article Title: Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation Article Snippet: Excess primers were removed by treating 50 μl PCR reactions with 3 μl of thermolabile exonuclease 1 (NEB, M0568L) for 4 min at 37°C before heat inactivating for 1 min at 80°C. .. Amplified DNA was then purified using a Monarch Spin PCR and Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs Article Snippet: .. For next generation sequencing (NGS), regions of interest were PCR-amplified and purified using a PCR and Article Title: Coordinated synthesis of double-stranded DNA by a dual reverse transcriptase immune system Article Snippet: Following lysis, DNA was purified with the Genomic DNA Clean & Concentrator-25 kit (Zymo). .. Following incubation at 55 °C for 7 minutes, the reaction was treated with 16 U mL −1 Proteinase K (NEB) and cleaned up with the Monarch Spin PCR and Article Title: Molecular insights into the dual activation of PomZ, a ParA/MinD P-loop ATPase, by the two ATPase-activating proteins PomX and PomY. Article Snippet: .. After purification with a Monarch Spin PCR and Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs. Article Snippet: .. For next generation sequencing (NGS), regions of interest were PCRamplified and purified using a PCR and Article Title: Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins HBS-N buffer 10X Cytiva Lifesciences #BR100828 MgCl 2 (1 M) InvitrogenTM #AM9530G Diethyl pyrocarbonate (DEPC) Bio Basic #DB0154 TritonTM X-100 Bio Basic #TB0198 Nuclease-Free Water (not DEPC-treated) Thermo Fisher ScientificTM #AM9937 Milli-Q water N/A N/A PhusionTM High-Fidelity DNA Polymerase New England Biolabs M0530S Deoxynucleotide (dNTP) Solution Mix New England Biolabs N0447S Critical commercial assays MEGAshortscriptTM T7 Transcription Kit InvitrogenTM AM1354 Monarch® Spin PCR and Article Title: Intragenic Transcription from Defective HIV Proviruses Triggers Interferon Responses in Myeloid Cells Article Snippet: .. SDS was used to denature the enzyme, then PCR cleanup performed to purify the annealed primers using Monarch Spin PCR and Article Title: Integration of ploidy, Orc1/Cdc6 homolog function, and lysine acetylation with phosphate limitation and UV stress responses of Haloferax volcanii. Article Snippet: .. PCR products of expected size were purified using Monarch PCR and Next-Generation Sequencing:Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs Article Snippet: .. For next generation sequencing (NGS), regions of interest were PCR-amplified and purified using a PCR and Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs. Article Snippet: .. For next generation sequencing (NGS), regions of interest were PCRamplified and purified using a PCR and Incubation:Article Title: Coordinated synthesis of double-stranded DNA by a dual reverse transcriptase immune system Article Snippet: Following lysis, DNA was purified with the Genomic DNA Clean & Concentrator-25 kit (Zymo). .. Following incubation at 55 °C for 7 minutes, the reaction was treated with 16 U mL −1 Proteinase K (NEB) and cleaned up with the Monarch Spin PCR and Binding Assay:Article Title: Molecular insights into the dual activation of PomZ, a ParA/MinD P-loop ATPase, by the two ATPase-activating proteins PomX and PomY. Article Snippet: .. After purification with a Monarch Spin PCR and Recombinant:Article Title: Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins HBS-N buffer 10X Cytiva Lifesciences #BR100828 MgCl 2 (1 M) InvitrogenTM #AM9530G Diethyl pyrocarbonate (DEPC) Bio Basic #DB0154 TritonTM X-100 Bio Basic #TB0198 Nuclease-Free Water (not DEPC-treated) Thermo Fisher ScientificTM #AM9937 Milli-Q water N/A N/A PhusionTM High-Fidelity DNA Polymerase New England Biolabs M0530S Deoxynucleotide (dNTP) Solution Mix New England Biolabs N0447S Critical commercial assays MEGAshortscriptTM T7 Transcription Kit InvitrogenTM AM1354 Monarch® Spin PCR and Software:Article Title: Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins HBS-N buffer 10X Cytiva Lifesciences #BR100828 MgCl 2 (1 M) InvitrogenTM #AM9530G Diethyl pyrocarbonate (DEPC) Bio Basic #DB0154 TritonTM X-100 Bio Basic #TB0198 Nuclease-Free Water (not DEPC-treated) Thermo Fisher ScientificTM #AM9937 Milli-Q water N/A N/A PhusionTM High-Fidelity DNA Polymerase New England Biolabs M0530S Deoxynucleotide (dNTP) Solution Mix New England Biolabs N0447S Critical commercial assays MEGAshortscriptTM T7 Transcription Kit InvitrogenTM AM1354 Monarch® Spin PCR and Spectrophotometry:Article Title: Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins HBS-N buffer 10X Cytiva Lifesciences #BR100828 MgCl 2 (1 M) InvitrogenTM #AM9530G Diethyl pyrocarbonate (DEPC) Bio Basic #DB0154 TritonTM X-100 Bio Basic #TB0198 Nuclease-Free Water (not DEPC-treated) Thermo Fisher ScientificTM #AM9937 Milli-Q water N/A N/A PhusionTM High-Fidelity DNA Polymerase New England Biolabs M0530S Deoxynucleotide (dNTP) Solution Mix New England Biolabs N0447S Critical commercial assays MEGAshortscriptTM T7 Transcription Kit InvitrogenTM AM1354 Monarch® Spin PCR and Fluorescence:Article Title: Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins HBS-N buffer 10X Cytiva Lifesciences #BR100828 MgCl 2 (1 M) InvitrogenTM #AM9530G Diethyl pyrocarbonate (DEPC) Bio Basic #DB0154 TritonTM X-100 Bio Basic #TB0198 Nuclease-Free Water (not DEPC-treated) Thermo Fisher ScientificTM #AM9937 Milli-Q water N/A N/A PhusionTM High-Fidelity DNA Polymerase New England Biolabs M0530S Deoxynucleotide (dNTP) Solution Mix New England Biolabs N0447S Critical commercial assays MEGAshortscriptTM T7 Transcription Kit InvitrogenTM AM1354 Monarch® Spin PCR and Membrane:Article Title: Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins HBS-N buffer 10X Cytiva Lifesciences #BR100828 MgCl 2 (1 M) InvitrogenTM #AM9530G Diethyl pyrocarbonate (DEPC) Bio Basic #DB0154 TritonTM X-100 Bio Basic #TB0198 Nuclease-Free Water (not DEPC-treated) Thermo Fisher ScientificTM #AM9937 Milli-Q water N/A N/A PhusionTM High-Fidelity DNA Polymerase New England Biolabs M0530S Deoxynucleotide (dNTP) Solution Mix New England Biolabs N0447S Critical commercial assays MEGAshortscriptTM T7 Transcription Kit InvitrogenTM AM1354 Monarch® Spin PCR and Sterility:Article Title: Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins HBS-N buffer 10X Cytiva Lifesciences #BR100828 MgCl 2 (1 M) InvitrogenTM #AM9530G Diethyl pyrocarbonate (DEPC) Bio Basic #DB0154 TritonTM X-100 Bio Basic #TB0198 Nuclease-Free Water (not DEPC-treated) Thermo Fisher ScientificTM #AM9937 Milli-Q water N/A N/A PhusionTM High-Fidelity DNA Polymerase New England Biolabs M0530S Deoxynucleotide (dNTP) Solution Mix New England Biolabs N0447S Critical commercial assays MEGAshortscriptTM T7 Transcription Kit InvitrogenTM AM1354 Monarch® Spin PCR and |
